형광염료(Fluorescence Dye)의 응용(영문)

형광염료(Fluorescence Dye)의 응용(영문) 형광염료(Fluorescence Dye)의 응용(영문).pptx 목차 1.Introduction 2.Types 3.Application 4.Conclusion 본문 Radioactivity 를 사용하는 것과의 비교 Cell 에서 특정단백질(actin)의 양을 알고 싶고, 세포 내에서 어떻게 되어있는지를 알고 싶어. 질문던지기 ) what do you do? 뭐 할 거냐?? 예전의 사람들, bioimaging tool을 모르는 사람들은 cell 을 깨고 antibody 를 쳐서 immunoprecipitation, western blotting을 하고 나온 gel 상의 밴드를 분석하고 그랬을 거다 But, as those who have learned bio-imaging, can you imagine a real situation in cells? 세포내부에서 번쩍거리는 실제 이미지를 상상할 수 있겠나? Like this – 다음 슬라이드 This picture shows an example, Detection of target proteins using fluorophore-conjugated antibodies. Synaptophysin (red) and microtubule-associated protein 2 (MAP2; green) in mouse cortical neurons were fluorescently labeled with specific primary antibodies and Thermo Scientific DyLight 649-Conjugated Goat Anti-Rabbit or DyLight 488-Conjugated Goat Anti-Mouse Secondary Antibodies, respectively. Nuclei in both panels were labeled with Hoechst dye. 본문내용 troduction Bioimaging group13. Live Cell Imaging I -Fluorescence Dye http://www.piercenet.com/browse.cfm?fldID=4DD9D52E-5056-8A76-4E6E-E217FAD0D86B#brightness Detection of target proteins using fluorophore-conjugated antibodies. 4 Introduction Live cell imaging Bioimaging group13. Live Cell Imaging I -Fluorescence Dye 5 Introduction Fluorescence Bioimaging group13. Live Cell Imaging I -Fluores 참고문헌 Valeur, Bernard. 2001. Molecular Fluorescence: Principles and Applications Wiley-VCH, p.29 http://www.piercenet.com/browse.cfm?fldID=4DD9D52E-5056-8A76-4E6E-E217FAD0D86B#introduction Loken MR (1990). Immunofluorescence Techniques in Flow Cytometry and Sorting (2nd ed.). Wiley. pp. 34153. Yasmin M Ramdzan, Tracking protein aggregation and mislocalization in cells with flow cytometry, Nature Methods (2012.3) Marion Cremer, Multicolor 3D Fluorescence In Situ Hybridization for Imaging Interphase Chromosomes , Methods Mol Biol, 2008 Retrieved on 2011-06-25. http://en.wikipedia.org/wiki/Fluorescence_in_the_life_sciences#Fluorescence_lifetime